Investigation of metabolic action of Cordyceps sinensis and its cultured mycelia on Escherichia coli by microcalorimetry / 药学学报

This study is to investigate the effect of Cordyceps sinensis and its cultured mycelia on growth and metabolism of Escherichia coli, and microcalorimetric method was carried out to evaluate its biological activity. The study will provide the basis for the quality control of Cordyceps sinensis. Experimental result will show the effect of natural Cordyceps sinensis and its cultured mycelia on growth and metabolism of Escherichia coli, with index of P(1max) and effective rate (E) by microcalorimetry, the data of experiment were studied by cluster analysis. The results showed that Cordyceps sinensis and its cultured mycelia not only can promote growth and metabolism of Escherichia coli but also can regulate the balance of intestinal microecology efficiently. When the concentrations of samples > 6.0 mg mL(-1), natural Cordyceps sinensis can promote the growth and metabolism of Escherichia coli efficiently (P < 0.05) compared with the control group, and have better dose-effect relationship with concentration (r > 0.9), its cultured mycelia does not show conspicuous auxoaction (P > 0.05) and have not dose-effect relationship with concentration (r < 0.6); when the concentration of samples < 6.0 mg mL(-1), all samples does not show conspicuous auxoaction (P > 0.05). Natural Cordyceps sinensis and its cultured mycelia can be distinguished by cluster analysis. Microcalorimetry has a good prospect on the quality evaluation of the traditional Chinese medicine.

HPLC fingerprint analysis of cordyceps and mycelium of cultured cordy / 中国中药杂志

<p><b>OBJECTIVE</b>To establish HPLC fingerprints of cordyceps and mycelium of cultured cordy for quality control.</p><p><b>METHOD</b>The analysis was carried out on a Diamonsil C18 column eluted with phosphate buffer (pH 6.5) and methanol as mobile phase, the gradient elution program was used, and the detection wavelength was 260 nm; The similarity of fingerprints was evaluated, then, cluster analysis was studied.</p><p><b>RESULT</b>The method of HPLC fingerprint analysis was validated and in keeping with the requirement. Ten common peaks were found in HPLC fingerprint. Cordyceps from different areas and mycelium of cultured cordy could be distinguished by the HPLC fingerprint.</p><p><b>CONCLUSION</b>All results above exhibit that this method is practicable, reproducible, and reliable as a method for the quality control of cordyceps and mycelium of cultured cordy at present.</p>